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Bioassays on FO probe surface. The surface of the gold-coated fiber-optic probe has a carboxyl monolayer to which capture antibodies are covalently bound. Target antibodies bind the capture antibodies in a label-free configuration (left). Alternatively, the signal can be enhanced by using gold nanoparticles functionalized with detection antibodies in a sandwich assay configuration (right) extending detection to the pM and fM range.

The FOx BIOSYSTEMS biosensor can accurately detect, quantify and measure the potency of your antibody of interest in a variety of sample matrices. Label-free pM to µM sensitivities in 10 minutes make our fiber-optic surface plasmon resonance (FO-SPR) biosensor a versatile development tool.

  • Fast assay time (10 min)
  • Clinically relevant sensitivity is in reach of either label-free or fast labeled amplification assays
  • Results better than ELISA, in much less time
  • Minimum sample preparation (serum, plasma and whole blood)

Case study:
Therapeutic drug monitoring

Infliximab (IFX) is a monoclonal antibody used for the treatment of inflammatory bowel disease. Optimizing the therapeutic outcome requires concentration measurements which are currently obtained using the slow ELISA or less sensitive lateral flow-based immunotherapy techniques.

Immediate dose adaptation is a challenge using these techniques because they require well-trained staff, a long time to result, and sample prep on serum samples.

FOx BIOSYSTEMS offers an accurate, fast and convenient alternative to currently used techniques. This study showed that the FO-SPR biosensor provided excellent agreement with ELISA, even for serum and plasma samples.

Antibody concentrations obtained with
FO-SPR correlate with those obtained with ELISA

Label-free potency screening of antibody–antigen interaction: FO-SPR can reproducibly measure and rank potency across a library.

Case study:
Potency screening

Quick, label-free quantification of antibodies reveals no information about their functionality, since they could be denatured but still detected. By adding a second step to bind the target of the antibody, the signal relative to the amount of antibody present is a strong indication of its performance. This allows fast and sensitive measurement and ranking of the antigen-binding potency of a set of antibodies.

Relevant publications

Found 14 Results
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App note: AN7 Versatile and fast IgG screening with the Protein A sensor probe

Application note 7 | Version 1.0 | Kris Ver Donck, Filip Delport, Laura Marin, Emma Buchet, Kim Stevens  SUMMARY FOx BIOSYSTEMS’ fiber-optic surface plasmon resonance technology (FO-SPR) is a robust…


March 20, 2023

Detection of Breast Cancer-Specific Extracellular Vesicles with Fiber-Optic SPR Biosensor

Yildizhan et al. (2023) International Journal of Molecular Sciences DOI: 10.3390/ijms24043764 Extracellular vesicles (EVs) have attracted great attention as potential biomarkers for cancer diagnostics. Although several technologies have been developed…


February 15, 2023

Innovative FO-SPR Label-free Strategy for Detecting Anti-RBD Antibodies in COVID-19 Patient Serum and Whole Blood

Qu et al. (2022) ACS Sensors DOI: 10.1021/acssensors.1c02215 The ongoing COVID-19 pandemic has emphasized the urgent need for rapid, accurate, and large-scale diagnostic tools. Next to this, the significance of…


January 27, 2022

App note: AN4 Label-free quantification in bioproduction samples

Application note 4 | Version 2 | Kris Ver Donck², Dagmara Minczakiewicz², Kim Stevens², Gert Struys¹, Thomas Geuens¹, Koen Dierckx¹, Filip Delport² ¹simAbs NV, ²FOx BIOSYSTEMS ABSTRACT This application note…


October 1, 2021

Gold nanoparticle enhanced multiplexed biosensing on a fiber optic surface plasmon resonance probe

Qu et al. (2021) Biosensors and Bioelectronics 192, 113549  We present an innovative multiplexing concept on a fiber optic surface plasmon resonance (FO-SPR) platform and demonstrate for the first time…


August 24, 2021

App note: AN3 Sensitive protein quantification in whole blood, dried blood spots, serum and plasma

Application note 3 | Version 1 | Kim Stevens, Kris Ver Donck, Filip Delport Based on original publication: Lu et al. (2017) Analytical Chemistry 89, 3664-3671. (1) ABSTRACT Fiber-optic surface…


June 29, 2021

App note: AN2 sensitive protein quantification in serum – comparison of FO-SPR and ELISA

Application note 2 | Kim Stevens, Kris Ver Donck, Filip Delport Based on original publication: Lu et al. (2016) Biosensors and Bioelectronics 79, 173–179. (1) ABSTRACT This application note demonstrates…


April 23, 2021

App note: AN1 carboxyl probe immobilization for label-free protein quantification

Application note 1 | Filip Delport, Dagmara Minczakiewicz, Kris Ver Donck ABSTRACT This application note describes a general strategy for optimizing immobilization on the FO-SPR probes and provides some reference…


March 31, 2021

Immunoassay for detection of infliximab in whole blood using a fiber-optic surface plasmon resonance biosensor

Lu et al. (2017) Anal. Chem. 89, 3664−3671   Monitoring the concentration of a therapeutic drug antibody, infliximab (IFX), is recommended for enhancing its efficacy in patients with inflammatory bowel…


February 22, 2017

Fiber optic-SPR platform for fast and sensitive infliximab detection in serum of inflammatory bowel disease patients

Lu et al. (2016) Biosensors and Bioelectronics 79, 173–179    Infliximab (IFX) is a therapeutic monoclonal antibody used for treating patients with inflammatory bowel disease (IBD). In order to improve…


May 15, 2016

Probing the force-induced dissociation of aptamer-protein complexes

Perez-Ruiz et al. (2014) Anal. Chem. 86, 3084−3091   Aptamers are emerging as powerful synthetic bioreceptors for fundamental research, diagnostics, and therapeutics. For further advances, it is important to gain…


February 20, 2014

Selection of aptamers against Ara h 1 protein for FO-SPR biosensing of peanut allergens in food matrices

Tran et al. (2013) Biosensors and Bioelectronics 43, 245–251    The rising prevalence to food allergies in the past two decades, together with the fact that the only existing therapy…


May 15, 2013

Fast and accurate peanut allergen detection with nanobead enhanced optical fiber SPR biosensor

Pollet et al. (2011) Talanta 83, 1436–1441   This paper is the first report of a fiber optic SPR biosensor with nanobead signal enhancement. We evaluated the system with a…


February 15, 2011

Fiber optic SPR biosensing of DNA hybridization and DNA–protein interactions

Pollet et al. (2009) Biosensors and Bioelectronics 25, 864–869   In this paper we present a fiber optic surface plasmon resonance (SPR) sensor as a reusable, cost-effective and label free biosensor for measuring DNA hybridization and DNA–protein…


December 15, 2009

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