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App note: Carboxyl probe immobilization for label-free protein quantification

By March 31, 2021No Comments

Application note 1 | Filip Delport, Dagmara Minczakiewicz, Kris Ver Donck

ABSTRACT

This application note describes a general strategy for optimizing immobilization on the FO-SPR probes and provides some reference example data as comparative guidance.

Surface plasmon resonance (SPR) is a powerful technique used to study a wide variety of label-free biomolecule interactions. However, there is no ‘one size fits all’ solution for ready-to-use applications and consumables that matches the variety of biomolecules used. Here, we describe a methodological approach to configure your specific biosensing surface on the generic carboxyl SPR probe for quantification purposes.

With this stepwise procedure, you can optimize the immobilization of a capture molecule, such as a specific monoclonal antibody, to obtain reproducible biosensors for quantifying your target antigen. This approach can be extended to other capture and target biomolecules, since SPR provides sensitive, label-free detection, irrespective of the type of binding interaction.

The flexibility to set up protocols and modify individual parameters from previously-used, stored protocols makes it easy to screen multiple condition variations, while real-time data visualization provides immediate feedback on your tests.

Furthermore, the unique FO-SPR probes can be dipped into the sample in a non-destructive method which only requires low volumes of precious samples which can be completely recovered afterwards, and also avoids clogging, thereby overcoming the limitations of microfluidic systems.

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