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The FOx BIOSYSTEMS instrument is effective in studying affinity and binding kinetics of proteins on large particles, such as microvesicles. The fluidics-free setup provides an advantage over existing biosensor platforms by eliminating the risk of clogging which is associated with complex targets, thereby removing the need for time-consuming rinsing procedures which may impact accuracy.

Case study:
Detecting extracellular vesicles

Extracellular vesicles are becoming increasingly important in the diagnosis, prognosis and therapy of many diseases, including cardiovascular disease and cancer. Their analysis is complicated by their range of size, composition and origin, compounded by the complexity of biofluids. Currently available methods have a range of downsides, including inaccuracy, complex/high-cost instruments or inability to process complex matrices.

The FOx BIOSYSTEMS instrument provides a fast and accurate method of detecting extracellular vesicles at physiologically relevant sensitivities. Its sensitivity is 103 – 104 times higher than that required for detection in healthy or cancer patients’ plasma. It was also shown to selectively capture breast cancer EVs spiked in blood plasma on the FO-SPR surface.

Extracellular vesicle detection. Capture antibodies covalently bound to the probe’s carboxyl monolayer bind the vesicle in a label-free configuration. The signal can be amplified using biotinylated detection antibodies and gold particles functionalized with anti-biotin antibodies.

Case study:
Detecting extracellular vesicles

FOx BIOSYSTEMS has received a grant from EIC accelerator to launch a collection of EV applications on WHITE FOxTM to the market in 2024, three years earlier than previously planned.

EV isolation and characterization is easy with WHITE FOxTM’s dip-in probe format that allows the specific binding, and release of EVs based on surface biomarkers.

Relevant publications

Found 5 Results
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White paper: WP9 Isolation of neuron-specific extracellular vesicles from human plasma using FO-SPR

White paper 9 | Version 2 | Tobias Zbik, Filip Delport SUMMARY Extracellular vesicles (EVs) are an increasingly popular target in the search for novel biomarkers in the field of...


January 25, 2024

Detection of Breast Cancer-Specific Extracellular Vesicles with Fiber-Optic SPR Biosensor

Yildizhan et al. (2023) International Journal of Molecular Sciences DOI: 10.3390/ijms24043764 Extracellular vesicles (EVs) have attracted great attention as potential biomarkers for cancer diagnostics. Although several technologies have been developed…


February 15, 2023

Immunogenic affinity and extracellular vesicle quantification in blood with FO-SPR

June 20-22, 2022 – MOSBRI Scientific Conference Our CTO, Dr. Filip Delport, had the opportunity to present at the 1st MOSBRI Scientific Conference in June 2022. He explained how the…


December 8, 2022

White paper: WP5 Extracellular vesicle quantification in complex matrices

White paper 5 | Version 2 | Kris Ver Donck, Filip Delport, Kim Stevens Based on original publication: Yildizhan et al. (2021) Journal of Extracellular Vesicles. 10 (4) ABSTRACT Fiber-optic...


December 15, 2021

FO-SPR biosensor calibrated with recombinant extracellular vesicles enables specific and sensitive detection directly in complex matrices

Yilidzhan et al. (2021) J. Extracell. Vesicles 10   Extracellular vesicles (EVs) have drawn huge attention for diagnosing myriad of diseases, including cancer. However, the EV detection and analyses procedures…


February 23, 2021

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For Research Use Only. Not for use in diagnostic procedures.