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Read our app note “Can you get immediate kinetic characterization from phage display clones?”


Read our app note “Improve your biopanning selection process with real-time monitoring”

FOx BIOSYSTEMS large particles applications icon

The FOx BIOSYSTEMS instrument is effective in studying affinity and binding kinetics of proteins on large particles, such as cells, viruses and microvesicles. The dip-in probe format even allows for biopanning, an affinity selection technique that is used to isolate phage displaying a peptide that binds to a given target.

Furthermore, the fluidics-free setup provides an advantage over existing biosensor platforms by eliminating the risk of clogging associated with cells and filamentous bacteria. In microfluidic instruments, this clogging necessitates time-consuming rinsing procedures and may impact accuracy.

Case study: Biopanning

M13 phage libraries expressing anti-eGFP binding peptides are grown, captured on eGFP funtionalised sensor probes and eluted for 3 culture cycles. The selection process is monitored with FO-SPR readout. The 3rd cycle individual clones are cultured for detailed characterization. FO-SPR real time binding sensorgrams of phage binding for the 3 subsequent biopanning rounds are shown (B-C-D).

Phage binding kinetics using fiber-optic SPR. Phage expressing eGFP receptors on coat proteins show differential SPR signals depending on whether the eGFP receptor is expressed on the P3 (A) or P8 (B) protein due to the difference in protein density on the phage surface.

Case study:
Bacteriophage kinetic affinity analysis

Increasing demands on biosensor sensitivity and specificity mean that multivalent bioreceptors are increasingly being used. In phage display, generating phage with high affinity to a product is an essential step. However, measuring the binding kinetics of complete phage particles is a challenge as these can clog microfluidics, albeit essential for the efficient design of these applications.

The FOx BIOSYSTEMS platform was used to study the affinity and binding kinetics of phage displaying peptide libraries in a microfluidic-free, dip-in experiment. A clear correlation was demonstrated between the SPR binding parameters and the expression of the target protein.

Relevant publications

Found 4 Results
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App Note: AN3 Improve your biopanning selection process with real-time monitoring

More efficient biotherapeutic discovery and screening using a fast, dip-in assay WHITE FOx can measure the real-time concentration of phage clones through multiple cycles of selection. The dipin sensor configuration...


May 14, 2024

App Note: AN2 Can you get immediate kinetic characterization from phage display clones?

More efficient biotherapeutic discovery and screening using a fast, dip-in assay WHITE FOx can measure the kinetics of phage clones to provide insights into their affinity and avidity, providing a...


White paper: WP6 Using FO-SPR to select for nanobodies in phage display

White paper 6 | Version 2 | Kris Ver Donck, Dagmara Minczakiewicz, Filip Delport, Kim Stevens Based on original publication: Knez et al. (2013) Analytical Chemistry 85, 10075 - 10082...


April 29, 2022

Affinity comparison of p3 and p8 peptide displaying bacteriophages using surface plasmon resonance

Knez et al. (2013) Anal. Chem. 85, 10075−10082   Ever increasing demands in sensitivity and specificity of biosensors have recently established a trend toward the use of multivalent bioreceptors. This…


September 30, 2013

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For Research Use Only. Not for use in diagnostic procedures.