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The FOx BIOSYSTEMS biosensor can accurately detect and quantify your target protein in a variety of sample matrices. Label-free pM to µM sensitivities in 10 minutes make our fiber-optic surface plasmon resonance (FO-SPR) biosensor a versatile development tool.

  • Fast assay time (10 min)
  • Clinically relevant sensitivity (0.97 – 80 ng/mL in 100x diluted sample)
  • Results better than ELISA, in much less time
  • Minimum sample preparation (serum, plasma and whole blood)

Bioassays on FO probe surface. The surface of the gold-coated fiber-optic probe has a carboxyl monolayer to which capture antibodies are covalently bound. Target proteins bind the capture antibodies in a label-free configuration (left). Alternatively, the signal can be enhanced by using gold nanoparticles functionalized with detection antibodies in a sandwich assay configuration (right) extending detection to the pM and fM range.

Case study: Immobilizing His-tagged bioreceptors

His tags are a commonly used method of selectively extracting proteins of interest from cells or display libraries.

A FO-SPR probe with NTA surface chemistry allows the oriented binding of such proteins for use as a bioreceptor, e.g. for display library screening. This chemistry is particularly convenient for using nanobodies and recombinant proteins, and also allows probe regeneration and reuse by easy removal of the bound protein.

The His tag binding has further benefits. Sequential analysis bioreceptor and immobilization followed by signal amplification using two different gold-labelled detection antibodies has been successfully demonstrated, allowing multiplexed target detection in buffer and 100-fold diluted human blood plasma.

Relevant publications

Found 12 Results
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White paper: WP8 Affinity determination of single domain antibodies for detection of biomarkers


White paper 8 | Version 2 | Laura Marin, Sarah Parmentier, Kris Ver Donck, Filip Delport SUMMARY Single domain antibodies (sdAbs) and adhirons are an alternative to conventional antibodies for...

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November 20, 2023


Measuring ADAMTS-13 activity to diagnose thrombotic thrombocytopenic purpura: a novel, fast fiber-optic surface plasmon resonance immunoassay


Bonnez et al. (2023) RPTH 7,  DOI: 10.1016/j.rpth.2023.102171 Thrombotic thrombocytopenic purpura (TTP) is characterized by severe ADAMTS-13 activity deficiency (<10%). Diagnostic testing is challenging because of unavailability, high cost, and…

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September 27, 2023


New technology for the development of molecular markers


Georg Johnen, Thomas Brüning, IPA (Institut für Prävention und Arbeitsmedizin der Deutschen Gesetzlichen Unfallversicherung) The detection of specific proteins is the focus of many methods for the diagnosis of occupational…

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December 7, 2022


Innovative FO-SPR Label-free Strategy for Detecting Anti-RBD Antibodies in COVID-19 Patient Serum and Whole Blood


Qu et al. (2022) ACS Sensors DOI: 10.1021/acssensors.1c02215 The ongoing COVID-19 pandemic has emphasized the urgent need for rapid, accurate, and large-scale diagnostic tools. Next to this, the significance of…

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January 27, 2022


White paper: WP4 Label-free quantification in bioproduction samples


White paper 4 | Version 4 | Kris Ver Donck², Dagmara Minczakiewicz², Kim Stevens², Gert Struys¹, Thomas Geuens¹, Koen Dierckx¹, Filip Delport² ¹simAbs NV, ²FOx BIOSYSTEMS ABSTRACT This white paper...

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October 1, 2021


Gold nanoparticle enhanced multiplexed biosensing on a fiber optic surface plasmon resonance probe


Qu et al. (2021) Biosensors and Bioelectronics 192, 113549  We present an innovative multiplexing concept on a fiber optic surface plasmon resonance (FO-SPR) platform and demonstrate for the first time…

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August 24, 2021


White paper: WP1 carboxyl probe immobilization for label-free protein quantification


White paper 1 | Filip Delport, Dagmara Minczakiewicz, Kris Ver Donck ABSTRACT This white paper describes a general strategy for optimizing immobilization on the FO-SPR probes and provides some reference...

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March 31, 2021


Probing the force-induced dissociation of aptamer-protein complexes


Perez-Ruiz et al. (2014) Anal. Chem. 86, 3084−3091   Aptamers are emerging as powerful synthetic bioreceptors for fundamental research, diagnostics, and therapeutics. For further advances, it is important to gain…

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February 20, 2014


Affinity comparison of p3 and p8 peptide displaying bacteriophages using surface plasmon resonance


Knez et al. (2013) Anal. Chem. 85, 10075−10082   Ever increasing demands in sensitivity and specificity of biosensors have recently established a trend toward the use of multivalent bioreceptors. This…

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September 30, 2013


Selection of aptamers against Ara h 1 protein for FO-SPR biosensing of peanut allergens in food matrices


Tran et al. (2013) Biosensors and Bioelectronics 43, 245–251    The rising prevalence to food allergies in the past two decades, together with the fact that the only existing therapy…

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May 15, 2013


Fast and accurate peanut allergen detection with nanobead enhanced optical fiber SPR biosensor


Pollet et al. (2011) Talanta 83, 1436–1441   This paper is the first report of a fiber optic SPR biosensor with nanobead signal enhancement. We evaluated the system with a…

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February 15, 2011


Fiber optic SPR biosensing of DNA hybridization and DNA–protein interactions


Pollet et al. (2009) Biosensors and Bioelectronics 25, 864–869   In this paper we present a fiber optic surface plasmon resonance (SPR) sensor as a reusable, cost-effective and label free biosensor for measuring DNA hybridization and DNA–protein…

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December 15, 2009


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For Research Use Only. Not for use in diagnostic procedures.