WHITE FOx biosensors can accurately detect and quantify your target protein in a variety of sample matrices. Label-free pM to µM sensitivities in 10 minutes make our fiber-optic biosensor a versatile development tool.
Bioassays on FO probe surface. The surface of the gold-coated fiber-optic probe has a carboxyl monolayer to which capture antibodies are covalently bound. Target proteins bind the capture antibodies in a label-free configuration (left). Alternatively, the signal can be enhanced by using gold nanoparticles functionalized with detection antibodies in a sandwich assay configuration (right) extending detection to the pM and fM range.
His tags are a commonly used method of selectively extracting proteins of interest from cells or display libraries.
A WHITE FOx probe with NTA surface chemistry allows the oriented binding of such proteins for use as a bioreceptor, e.g. for display library screening. This chemistry is particularly convenient for using nanobodies and recombinant proteins, and also allows probe regeneration and reuse by easy removal of the bound protein.
The His tag binding has further benefits. Sequential analysis bioreceptor and immobilization followed by signal amplification using two different gold-labelled detection antibodies has been successfully demonstrated, allowing multiplexed target detection in buffer and 100-fold diluted human blood plasma.
White paper 8 | Version 2 | Laura Marin, Sarah Parmentier, Kris Ver Donck, Filip Delport SUMMARY Single domain antibodies (sdAbs) and adhirons are an alternative to conventional antibodies for...
November 20, 2023
Bonnez et al. (2023) RPTH 7, DOI: 10.1016/j.rpth.2023.102171 Thrombotic thrombocytopenic purpura (TTP) is characterized by severe ADAMTS-13 activity deficiency (<10%). Diagnostic testing is challenging because of unavailability, high cost, and…
September 27, 2023
Georg Johnen, Thomas Brüning, IPA (Institut für Prävention und Arbeitsmedizin der Deutschen Gesetzlichen Unfallversicherung) The detection of specific proteins is the focus of many methods for the diagnosis of occupational…
December 7, 2022
Qu et al. (2022) ACS Sensors DOI: 10.1021/acssensors.1c02215 The ongoing COVID-19 pandemic has emphasized the urgent need for rapid, accurate, and large-scale diagnostic tools. Next to this, the significance of…
January 27, 2022
White paper 4 | Version 4 | Kris Ver Donck², Dagmara Minczakiewicz², Kim Stevens², Gert Struys¹, Thomas Geuens¹, Koen Dierckx¹, Filip Delport² ¹simAbs NV, ²FOx BIOSYSTEMS ABSTRACT This white paper...
October 1, 2021
Qu et al. (2021) Biosensors and Bioelectronics 192, 113549 We present an innovative multiplexing concept on a fiber optic surface plasmon resonance (FO-SPR) platform and demonstrate for the first time…
August 24, 2021
White paper 1 | Filip Delport, Dagmara Minczakiewicz, Kris Ver Donck ABSTRACT This white paper describes a general strategy for optimizing immobilization on the FO-SPR probes and provides some reference...
March 31, 2021
Perez-Ruiz et al. (2014) Anal. Chem. 86, 3084−3091 Aptamers are emerging as powerful synthetic bioreceptors for fundamental research, diagnostics, and therapeutics. For further advances, it is important to gain…
February 20, 2014
Knez et al. (2013) Anal. Chem. 85, 10075−10082 Ever increasing demands in sensitivity and specificity of biosensors have recently established a trend toward the use of multivalent bioreceptors. This…
September 30, 2013
Tran et al. (2013) Biosensors and Bioelectronics 43, 245–251 The rising prevalence to food allergies in the past two decades, together with the fact that the only existing therapy…
May 15, 2013
Pollet et al. (2011) Talanta 83, 1436–1441 This paper is the first report of a fiber optic SPR biosensor with nanobead signal enhancement. We evaluated the system with a…
February 15, 2011
Pollet et al. (2009) Biosensors and Bioelectronics 25, 864–869 In this paper we present a fiber optic surface plasmon resonance (SPR) sensor as a reusable, cost-effective and label free biosensor for measuring DNA hybridization and DNA–protein…
December 15, 2009
For Research Use Only. Not for use in diagnostic procedures.
