Flexible bioassays – protein, DNA, RNA

FOx BIOSYSTEMS flexible applications icon

Fiber-optic surface plasmon resonance (FO-SPR) is a versatile technology that can be adapted for use in a wide variety of additional applications.

Our interchangeable probes are available with four surface chemistries, allowing you to select the most appropriate method for your bioassay.

Carboxyl

A generic surface chemistry that serves a wide range of applications

NTA

For immobilized His-tag proteins; convenient for nanobodies and recombinant proteins and ideal for display library screening; allows multiplexing

Streptavidin

For easy immobilization of biotin-labelled proteins, antibodies or peptides

Protein A

A generic surface for quantifying IgG; ideal for screening IgG libraries for binding potency

This combination of probes allows the quantification of proteins, small molecules, DNA or cells in microliter volumes, as well as a wide range of interaction analyses.

Furthermore, the fluidics-free setup also eliminates the clogging associated with microfluidics-based alternatives, thereby reducing sample processing steps.

See the publication list for more examples of what scientists are doing with our technology.

Some of the other exciting things scientists are using our technology for:

Biologics production
- Nanobody- or antibody-antigen kinetic affinity
- Cellular bioreactor development
- In-line process control systems
- Analyzing antibody levels and binding kinetics in patient blood
Extracellular vesicle (EV) characterization
Fast sandwich assays using gold nanoparticles
Potency / denaturation assays
DNA / RNA
- Quantification
- Hybridization
- And more
Allergen testing in food
- Peanut
- Progesterone in dairy milk
A wide range of interaction analyses, including potency assays, enzymatic lysis, etc.

The FO-SPR probes can be coupled with a range of compounds. Make use of the flexibility provided by interchangeable probes with different surfaces chemistries.

The FOx instrument features flexible bioassay protocols that can be adapted to your target molecule.

See the publication list for more examples of what scientists are doing with our technology.

Relevant publications

Integrated Signal Amplification on a Fiber Optic SPR Sensor Using Duplexed Aptamers

Real-time FO-SPR monitoring of solid-phase DNAzyme cleavage activity for cutting-edge biosensing

Competitive inhibition assay for the detection of progesterone in dairy milk using a fiber optic SPR biosensor

Real-time ligation chain reaction for DNA quantification and identification on the FO-SPR

Probing the force-induced dissociation of aptamer-protein complexes

Selection of aptamers against Ara h 1 protein for FO-SPR biosensing of peanut allergens in food matrices

Fiber optic SPR biosensing of DNA hybridization and DNA–protein interactions

Challenge us to help you with your application!