White paper 10 | Version 1 | Tobias Zbik, Sarah Parmentier, Kris Ver Donck
ABSTRACT
The landscape of antibody research continuously evolves, with novel antibody variants such as VHH, antibody-drug conjugates, and bivalent antibodies emerging with huge potential in drug development. This white paper explores the kinetic analysis of VHH and a human bivalent antibody using FO-SPR technology. We showcase how the remarkable sensitivity of the SPR dip-in method enables the measurement of high-affinity kinetics, even for low molecular weight antibodies like VHH. Furthermore, real-time measurements and flexibility in assay design provide a means to address challenging experimental designs, particularly relevant for studying bivalent antibodies.