Our customizable, interchangeable probes consist of a gold-coated, optical fiber with a choice of four different surface chemistries that allow the binding of a range of proteins and biologics. These disposable probes are placed in the White FOx, a high-end, semi-automated analytical benchtop instrument for real-time analysis of biomolecular interactions.

This sensor technology uses the fiber-optic surface plasmon resonance (FO-SPR) principle for both labelled and label-free detection of biomolecules, including proteins, antibodies, nanobodies, phage, complex particles, microvesicles, or cells in microliter volumes.

Within a few minutes, you can get accurate information on binding affinity, kinetics or target concentration.

These interchangeable probes come in four different formats meaning that the White FOx can detect different target molecules with a single device, giving you full flexibility in use.

Our probes are now available with four different surface chemistries, allowing you to select the optimal method for your bioassay. All formats can be shipped and stored at room temperature and can be regenerated for reuse after most types of assays.

Carboxyl
A generic surface chemistry that serves a wide range of applications
NTA
For immobilized His-tag proteins; convenient for nanobodies and recombinant proteins and ideal for display library screening; allows multiplexing
Streptavidin
For easy immobilization of biotin-labelled proteins, antibodies or peptides
Protein A
A generic surface for quantifying IgG; ideal for screening IgG libraries for binding potency
Additional probe types are in development that will open up an even broader range of applications.

human IgG(1) concentration series from 0; 0.03 to 256 µg/ml, captured on a Protein A probe, over >45 cycles of regeneration.

Regeneration

All probes are compatible to common regeneration conditions. While regeneration on carboxyl and streptavidin probes may be limited by the stability of the capture proteins immobilised in the assay, the NTA and protein A probes allow for complete regeneration to return to the original probe surface. As these surfaces are robust, many regeneration cycles are possible.

In fast IgG quantification, more than 45 cycles of regeneration have shown to still produce complete recovery of probe sensitivity. So you can screen efficiently and reliably across an entire assay plate.

Contact us to find out more about these exciting developments!

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