Skip to main content

Biopanning is a technique used to select peptides or antibodies that bind to a target of interest using affinity selection. Phage are engineered to display a peptide of interest and captured by conjugating to a desired target.

The FO-SPR probes of WHITE FOxTM are ideally suited for this procedure. The user-friendly dip-in, fluidics-free setup offers a simple protocol for the binding, washing and elution steps, and the binding affinity, association and dissociation kinetics of the phage can be measured in real time. Unbound phage are washed from the surface and, finally, the bound phage are eluted to obtain the desired high-affinity phage clones which can then be used to create further display libraries.

Bacteriophage kinetic affinity analysis

Generating phage with high affinity to a product is an essential step in the development and production of many proteins and antibodies. A method that can both select the phage with highest affinities and also measure the binding and dissociation kinetics is highly valuable in the efficient design of these applications. However, typical methods for measuring binding kinetics rely on microfluidics and are prone to clogging by phage particles.

The FOx BIOSYSTEMS platform was used to select phage with high affinity for eGFP in order to study the affinity and binding kinetics of phage displaying peptide libraries in a microfluidic-free, dip-in experiment. A clear correlation was demonstrated between the SPR binding parameters and the expression density of the target protein and two phage clones with dissociation constants in the femtomolar range were found.

Affinity selection of phage fiber-optic SPR. Phage expressing eGFP receptors on coat proteins were analyzed for their affinity for eGFP. Furthermore, differential SPR signals were obtained when the eGFP receptor was expressed on the P3 (A) or P8 (B) protein due to the difference in protein density on the phage surface.

Case study:
Detecting extracellular vesicles

FOx BIOSYSTEMS has received a grant from EIC accelerator to launch a collection of EV applications on WHITE FOxTM to the market in 2024, three years earlier than previously planned.

EV isolation and characterization is easy with WHITE FOxTM’s dip-in probe format that allows the specific binding, and release of EVs based on surface biomarkers.

Relevant publications

Found 4 Results
Page 1 of 1

App Note: AN3 Improve your biopanning selection process with real-time monitoring

More efficient biotherapeutic discovery and screening using a fast, dip-in assay WHITE FOx can measure the real-time concentration of phage clones through multiple cycles of selection. The dipin sensor configuration...

May 14, 2024

White paper: WP6 Using FO-SPR to select for nanobodies in phage display

White paper 6 | Version 2 | Kris Ver Donck, Dagmara Minczakiewicz, Filip Delport, Kim Stevens Based on original publication: Knez et al. (2013) Analytical Chemistry 85, 10075 - 10082...

April 29, 2022

WHITE FOxTM technical specifications

You can find the technical specifications of the White FOxTM here: DOWNLOAD

April 8, 2021

Affinity comparison of p3 and p8 peptide displaying bacteriophages using surface plasmon resonance

Knez et al. (2013) Anal. Chem. 85, 10075−10082   Ever increasing demands in sensitivity and specificity of biosensors have recently established a trend toward the use of multivalent bioreceptors. This…

September 30, 2013

Page 1 of 1

Contact us today to see how we can help you.

Contact us

For Research Use Only. Not for use in diagnostic procedures.