Biopanning is a technique used to select peptides or antibodies that bind to a target of interest using affinity selection. Phage are engineered to display a peptide of interest and captured by conjugating to a desired target. Unbound phage are washed from the surface and, finally, the bound phage are eluted to obtain the desired high-affinity phage clones which can then be used to create further display libraries.
The FO-SPR probes of the White FOx are ideally suited for this procedure. The user-friendly dip-in, fluidics-free setup offers a simple protocol for the binding, washing and elution steps, and the binding affinity, association and dissociation kinetics of the phage can be measured in real time.