When developing both diagnostic methods and therapies, understanding complex protein interactions, especially those of antibodies, is crucial.
WHITE FOx is a simple-to-use characterization tool and requires minimal sample preparation which, combined with a fast response time and automation, makes it a flexible and reliable solution for life science research and bioprocess monitoring.
With this simple, dip-in process, you can rank affinities of proteins, antibodies, antigens, single domain antibodies, bi- or multispecific antibodies, biomarkers, receptors and ligands, and measure bioreceptor-target binding kinetics, affinity (kD) and on & off rates (Kon, Koff), all label free.
Case study: Kinetic analysis of novel antibody variants
Antibody research is continuously evolving, through the development of novel variants such as VHH, antibody-drug conjugates, and bivalent antibodies. Characterizing these new types of molecules requires innovative methods.
WHITE FOx provides a flexible dip-in SPR method to measure high-affinity binding kinetics, also for smaller proteins like VHH.
Kinetic analysis of anti-rabbit VHH binding to rabbit IgG. FO-SPR provides association and dissociation curves which can be used to obtain kon and koff.
Kinetic profile of a high affinity clone selected from biopanning against eGFP. This clone had a kD of 1.2×10-14 M on WHITE FOx while it was missed due to scoring low on the end-point method ELISA.
Kinetic analysis in phage display workflow
Phage display with biopanning is often used to select single domain antibodies and other proteins specifically binding to a target antigen. Being able to both monitor polyclonal biopanning cycles, as well as characterize binding kinetics directly on the monoclonal phage in crude samples, greatly simplifies the process of selecting the best clones.
Relevant publications
Found 9 Results
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White paper: WP8 Affinity determination of single domain antibodies for detection of biomarkers
November 20, 2023
Detection of Breast Cancer-Specific Extracellular Vesicles with Fiber-Optic SPR Biosensor
February 15, 2023
New technology for the development of molecular markers
December 7, 2022
Innovative FO-SPR Label-free Strategy for Detecting Anti-RBD Antibodies in COVID-19 Patient Serum and Whole Blood
January 27, 2022
WHITE FOxTM technical specifications
April 8, 2021
Affinity comparison of p3 and p8 peptide displaying bacteriophages using surface plasmon resonance
September 30, 2013
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For Research Use Only. Not for use in diagnostic procedures.