Bioassays on FO probe surface. The surface of the gold-coated fiber-optic probe has a carboxyl monolayer to which capture antibodies are covalently bound. Target antibodies bind the capture antibodies in a label-free configuration.
The FOx BIOSYSTEMS biosensor can accurately detect, quantify and measure the potency of your target of interest in a variety of sample matrices. Label-free nM to µM sensitivities in as little as one minute make our fiber-optic surface plasmon resonance (FO-SPR) biosensor a versatile development tool.
- Fast assay time (from 1 to 20 min)
- Clinically relevant sensitivity (0.97 – 80 ng/mL in 100x diluted sample)
- Results better than ELISA, in much less time
- Minimum sample preparation (serum, plasma and whole blood)
hIgG screening.
The protein A sensor probe allows for fast label free concentration screening of total hIgG in cell culture samples. With a fast assay time, down to 1 minute, and ng/ml to µg/ml sensitivity with a wide dynamic range with excellent reproducibility this is an ideal tool for rapid screening.
hIgG concentration example curve on protein A probes. Triplicate data points shown, with CVs well below 5%.
Excellent repeatability. Calibration curves obtained from plotting the SPR shift against a function of the concentration gave almost identical results when performed five weeks apart.
Case study:
Label-free quantification in bioproduction samples
Monitoring the production of antibodies is crucial for understanding and optimizing the process. In this study, production of a therapeutic monoclonal antibody was used to investigate a new method to monitor production in real time.
ELISA is the most commonly used protein detection method, but this is a slow technique and is typically run in batches of samples collected over time, greatly increasing the time to result.
WHITE FOxTM from FOx BIOSYSTEMS offers a fast and convenient alternative to ELISA, giving comparable results within 10 minutes when using pre-functionalized probes. The dip-in protocol means that even individual samples can be analyzed straight after collection, directly in the sample medium, allowing for a rapid turnaround that facilitates near-real-time process optimization.
Relevant publications
Found 14 Results
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Detection of Breast Cancer-Specific Extracellular Vesicles with Fiber-Optic SPR Biosensor
February 15, 2023
New technology for the development of molecular markers
December 7, 2022
Innovative FO-SPR Label-free Strategy for Detecting Anti-RBD Antibodies in COVID-19 Patient Serum and Whole Blood
January 27, 2022
WHITE FOxTM technical specifications
April 8, 2021
FO-SPR biosensor calibrated with recombinant extracellular vesicles enables specific and sensitive detection directly in complex matrices
February 23, 2021
Probing the force-induced dissociation of aptamer-protein complexes
February 20, 2014
Affinity comparison of p3 and p8 peptide displaying bacteriophages using surface plasmon resonance
September 30, 2013
Selection of aptamers against Ara h 1 protein for FO-SPR biosensing of peanut allergens in food matrices
May 15, 2013
Fast and accurate peanut allergen detection with nanobead enhanced optical fiber SPR biosensor
February 15, 2011
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For Research Use Only. Not for use in diagnostic procedures.